The goals of this single-blinded clinical study are to evaluate the efficacy of Follione Hair Loss Serum for Women in improving the hair thickness, hair density and hair growth after a 56-day twice-daily treatment, in a dermatological controlled in vivo study, to evaluate the subjects’ tolerance, acceptability, perception of efficacy and intention of future use/purchase regarding the cosmetic products by filling in a subjective evaluation questionnaire after 56 days of treatment and to evaluate the clinical signs and symptoms of cutaneous irritation by an expert 56 days after the beginning of the treatment.

In order to perform this study, 33 healthy female subjects within 20 to 55 years old also presenting early stages of alopecia are enrolled. The subjects will be testing one investigational product.

The investigational product is applied in the most affected area of the subjects, previously selected by the Principal Investigator or its designee, twice daily, in mornings and evenings, during a 56-day period. A shampoo without any kind of anti-hair loss effect is provided to each subject to use throughout the study as a non-investigational product, applying it with the normal frequency of use.

To visualize the evolution of the hair during the treatment, high-resolution photographs of the superior part of the head are taken with the equipment Visioface® 1000 D (Courage+Khazaka electronic GmbH, Germany), in standardized lighting conditions and positions, before and after 56 days of the application of the product.

The efficacy of the cosmetic product is evaluated through biometric measurements, namely hair growth, hair density, and hair thickness, assessed before and 56 days after the beginning of the treatment. These parameters are assessed with the equipment Trichoscan® Smart (Datinf, Germany) on a previously selected shaved scalp area. An expert classifies the tolerance to the treatment, using clinical signs and symptoms classifying scales, 56 days after the beginning of the treatment. Subjects also fill in a subjective evaluation questionnaire regarding the product acceptability, tolerance, efficacy and intention of future use/purchase after 56 days of the beginning treatment. The entire study is supervised by a dermatologist (M.D.).

The most relevant results that can be concluded from this study are:

After 56 days of treatment, a statistically significant mean increase on the hair thickness up to 66.54% was observed.
This is in accordance with the subjects’ opinion once 73.33% of the subjects considered that the product provides a slight to very strong hair thickening and strengthening effect.

Similar results were obtained for the hair density, with a statistically significant mean increase up to 77.61% after the treatment.
76.67% of the subjects mentioned that the product provides a slight to very strong stimulating effect.
73.33% of the subjects mentioned that the product provides a slight to very strong hair growth rhythm acceleration effect.

DIAMINOPYRIMIDINE OXIDE 1.5%
It is a clinically proven hair growth stimulant. Diaminopyrimidine oxide helps to increase hair size and volume.

LITSEA GLUTINOSA LEAF EXTRACT 1%
Brevilin A is a natural JAK-STAT Inhibitor found in LITSEA GLUTINOSA.

CAPILIA LONGA 0.5%
CAPILIA LONGA PPF acts as a Hair 3D Bio-Printer, resetting and guiding the hair follicle growth with a bio-software (Phyto-Peptidic Fractions), nourishing at the same time the hair follicle growth with an innovative Bio-Ink (Exclusive Nutritive Blend)

BLACK CUMIN SEED EXTRACT 0.25%
Thymoquinone is a phytochemical compound found in the plant Nigella sativa (Black Cumin seed extract). It has anti-inflammatory properties.

TROXERUTIN 1000ppm
Troxerutin is a rutoside, a naturally occurring flavonoid. It protects HDP cells against the H2O2-mediated reduction in viability. HDP cells are a major component of skin and direct hair growth, and loss or senescence of these cells is a key cause of hair loss.

CHLOROPHYLL 700ppm
Chlorophyllin has been shown to have and antioxidant properties, inhibiting DNA adduction. Chlorophyllin also quenches all major oxygen species and acts to protect mitochondria. It provides a safe source of copper.

BAICALIN 500ppm
Baicalin, a flavonoid isolated from Scutellaria baicalensis, is known to have multiple biological functions. It activates Wnt/?-catenin signaling in a dose-dependent manner in human DP cells. Results strongly suggest that baicalin promotes hair growth by regulating the activity of DP cells.

WEDELOLACTONE 500ppm
Wedelolactone is a plant-derived natural product synthesized mainly by members belonging to the Asteraceae family. It has hair growth promoting activity. Clinical results indicate that plant extract with suitable vehicle may be useful as an alternative topical medicine to minoxidil therapy.

FISETIN 50ppm
Fisetin, a flavonol found in strawberries inhibits UVB-induced cutaneous inflammation.

RASPBERRY KETONES 40ppm
Topical application of raspberry ketones increases immunohistochemical expression of IGF-I at dermal papillae in hair follicles, promotes hair re-growth and increases skin elasticity.

SOY ISOFLAVONES 10ppm
Soy isoflavones play an important role in slowing down the aging process of the scalp, while their effect on epidermal and fibroblast cells dramatically boost elastin and collagen production.

FORMONONETIN 10ppm
Formononetin is one of the main components of red clover plants. Topical formononetin treatment induces miniaturized hair follicles to recover to normal sizes.

BIOCHANIN-A 10ppm
Biochanin-A is one of the main components of red clover plants. It is a natural inhibitor of DHT, subdues hair loss, and modulates free radical damage to the skin and scalp.

COPPER TRIPEPTIDE-1 10ppm
It stimulates skin repair and increases the number of subcutaneous fat cells in the scalp, which strengthens and increases the size of hair follicles.

Application:

Shake well before use. Apply 1 ml of the substance to the area being treated with the pipette or nozzle provided in the package. Gently rub the liquid into your scalp and let it dry. Do not wash your hair within three hours of treatment. Repeat the application twice a day, mornings and evenings. To maintain the achieved results, continue to use the product once a day. For best results, use the product with Follione^® Anti-Hair Loss Liquid Shampoo.

Other information:

• Store at room temperature.
• After use, close the cap tightly.

Warnings:

For external use only. In case of contact with the eyes, rinse thoroughly with water. Keep away from children. Do not apply to open wounds! Discoloration and fine deposits can occur in the product due to the presence of herbal ingredients, but these do not influence the effectiveness and usability of the product.

Hair regrowth does not occur overnight and it does not happen in the same way for everyone. Using any legitimate hair growth product will require consistent treatment for six to twelve months or longer. The most important thing you can do is get started early and don’t wait until you are bald to take a stand against hair loss solution for men.

Anyone who tells you to look for signs of growth overnight is not being honest with you. With Follione, we make no such promises. We know that it takes time and patience. We also know that it definitely works if you use the treatment consistently as directed.

1-2 MONTHS
You may not see it growing, but it is. There are invisible changes that must take place within your scalp before you notice visible hair growth.

3-5 MONTHS
Almost all users experience hair loss stabilization, within four months when they use the products consistently.

6-12 MONTHS
Toward the end of month 6-12 during the hair regrowth process, you may begin seeing more visible hair regrowth in thinning areas, as well as thinning hair beginning to thicken. Patches of the exposed scalp will become less visible as new, thicker hair begins to fill in.

Are there any side effects using your hair loss treatments and products?
All Follione hair loss treatments and products are safe, effective, and there are no side effects.

Are the Follione products drugs, and are they approved?
Follione products are not drugs, and all the ingredients of the Follione products are approved.

Do the Follione hair loss products contain Minoxidil or Finasteride?
Minoxidil is the drug that is in Rogaine, Avacor, and Scalpmed. Finasteride is the drug that is present in Propecia and Proscar. Neither of these drugs is present in Follione formulas. Rather than focusing on hair loss prevention, all of these drug-based products tend to override problems and provide a positive response in a low percentage of users.

Does your hair loss treatment cause temporary hair loss before the benefits kick in?
Hair fall should not be confused with hair loss. The Follione hair loss treatment is designed to create the best possible environment within the hair follicles, where the natural cycles of hair fall and hair replacement take place. Sometimes, new clients experience a little more hair fall than usual for the first few weeks. This is a normal result of the increased cleansing taking place, along with an improvement in the scalp’s metabolism.

Why do the other hair loss treatments and minoxidil solutions feel so oily?
Rogaine and the generic minoxidil solutions contain a 50% concentration of Propylene Glycol in their liquid base, which leaves a long-lasting greasy film on the scalp. One Green Lab uses no PPG to help reduce the greasy feeling.